Optimization of Bacterial Cellulose-Quercetin Biocomposite Synthesis Using The Ex-Situ Method and Its Application as an Antibacterial Agent

2025-03-07T04:19:30+00:00

Bacterial cellulose is a natural polymer synthesized by bacteria, possessing significant potential for biomedical and pharmaceutical applications. However, it lacks inherent antibacterial properties, necessitating modification with additional materials. Quercetin, a flavonoid compound, is known for its pharmacological activities, including antibacterial effects by disrupting bacterial cell walls. This study aims to identify the antibacterial activity of bacterial cellulose-quercetin biocomposite by optimizing the concentration and modification time, and to characterize their functional groups. Bacterial cellulose was modified using an ex-situ method by immersing it in quercetin solution. Antibacterial testing was performed using the disk diffusion method against Staphylococcus aureus and Escherichia coli. Results indicated that the optimal bacterial cellulose-quercetin biocomposite was achieved at a concentration of 6% with a modification time of 48 hours, showing excellent antibacterial properties with inhibition zones of 18.5 mm for S. aureus and 18 mm for E. coli. FTIR analysis confirmed the main functional groups of cellulose (O-H, C-H, and C-O-C glycosidic bonds) in both bacterial cellulose and the composite, showing no significant changes after immersion in distilled water. This suggests that quercetin binds strongly to the bacterial cellulose matrix, resulting in a stable biocomposite with potential as an effective antibacterial material, such as for wound healing.

Characterization of Simplicia, Extract, and Mineral Content of Broccoli (Brassica oleracea var. italica)

2025-05-20T13:55:06+00:00

Bioactive compounds and minerals in medicinal plants make them both therapeutic agents and raw materials for producing contemporary medications. Broccoli (Brassica Oleracea var Italica) is one of the possible therapeutic plants that features several nutrients that are high in minerals. This study produced Broccoli ethanol extract and characterized the water, ash, and phytochemical test of simplicia and extract. The mineral content of simplicia, such as calcium, potassium, sodium, and iron, used the flame test Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) method. This method is specifically for analyzing atomic elements or ions, which causes them to be excited and emit light at specific wavelengths. The result of water and ash content of Broccoli simplicia and ethanol extract within a range of values 2-9 – 5.8 %. Positive results for alkaloids, flavonoids, saponins, and tannins in phytochemical tests of simplicia and ethanol extract. The mineral content showed that calcium, potassium, sodium, and iron were 109.52 ± 0.25 mg/100 g, 375.58 ± 0.81 mg/100 g, 9.54 ± 0.092 mg/100 g, and 1.93 ± 0.00 mg/100g, respectively. It was concluded that as a natural ingredient, broccoli contains higher potassium than other minerals, and secondary metabolites in simplex and ethanol extracts have health benefits.

Helium: Journal of Science and Applied Chemistry

Optimization of Bacterial Cellulose-Quercetin Biocomposite Synthesis Using The Ex-Situ Method and Its Application as an Antibacterial Agent

Characterization of Simplicia, Extract, and Mineral Content of Broccoli (Brassica oleracea var. italica)